Detection of rice syrup content in honey requires advanced equipment. A sample of the purified honey liquid was sent to a liquid chromatography-tandem mass spectrometer. The experimenter explained that if a column is used as a runway, different substances can separate different sugars by the polarity of the liquid phase. Due to the difference in molecular weight and molecular structure polarity, they will be driven equally. Through chromatographic observation, the time budget for different substances to reach the peak can determine whether it is rice syrup, and the area reaching the peak can determine the content of rice syrup.

How to detect the content of rice syrup in honey

SM-R is a unique substance in rice syrup. The purity of honey is also one of the important and basic testing items. It is one of the compulsory inspection items for China's export of EU honey. If the product is positive for SM-R, it is suspected of containing rice syrup in honey. Although rice syrup is also sugar, it is cheap and has completely different health benefits.

Β-fructofuranosidase detection

Β- Test for fructofuranosidase on a liquid chromatograph. After polar separation, the same sample was sent to a control group using a standard chromatograph to determine if it contained β-fructofuranosidase.

Β-fructofuranosidase can directly convert sucrose into glucose and fructose. As one of the means of adulteration of honey, its mechanism of action is to break the s- (1,4) glycosidic bond between glucose and the sugar group of ordinary cane candy, and produce fructose and glucose. β-fructofuranosidase and disaccharide sucrose are added at the same time. Sucrose can be directly converted into glucose and fructose. 90% of the components in natural honey are glucose and fructose. Chemically produced "honey" has completely different nutritional values from natural honey.

According to the above introduction, the rice syrup content in honey can be detected.